Anesthesia & Analgesia
Injectable Anesthesia for Laboratory Animals
Drug(s) |
Mouse |
Rat |
Rabbit |
Ketamine:Xylazine |
80-120 mg/kg:10-16 mg/kg IP (20-40 min. of anesthesia) |
80-100 mg/kg: 5-10mg/kg IP (20-50 min. of anesthesia) |
22-50 mg/kg:2.5-10 mg/kg IM (25-40 minutes of anesthesia) |
Ketamine:Xylazine: Acepromazine |
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50 mg/kg: 5 mg/kg: 1 mg/kg IP (30-45 minutes of anesthesia) |
35 mg/kg: 5 mg/kg: 0.75 mg/kg IM (45-75 min. of anesthesia) |
Ketamine:Medetomidine |
75 mg/kg: 1.0 mg/kg IP (20-30 min. of anesthesia) |
75 mg/kg: 0.5 mg/kg IP (20-30 min. of anesthesia) |
25 mg/kg: 0.5 mg/kg IM (30-40 min. of anesthesia) |
Ketamine:Valium - short duration, light anesthesia |
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5 mg/kg : 0.25 mg/kg IV.
Mix equal volumes of ketamine (100 mg/ml) and valium ( 5 mg/ml). Dosage rate: 1 ml per 20 lbs body weight. |
Inactin (thiobutabarbital, EMTU) |
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80-100 mg/kg IP (60-240 min. of anesthesia) |
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Pentobarbital -variable anesthetic depth; poor analgesia |
30-50 mg/kg IP (20-40 min. of anesthesia) |
40-50 mg/kg IP (20-60 min. of anesthesia) |
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Tribromoethanol (Avertin) |
240 mg/kg IP (15-45 min. of anesthesia) |
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Atipamezole (for reversal of Xylazine or medetomidine) |
1.0 mg/kg IP |
1.0 mg/kg IP |
0.1-1.0 mg/kg IM, IP, SQ or IV (dose required depends on dose of xylazine or medetomidine administered) |
Glycopyrrolate (preanesthetic) |
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0.1 mg/kg IM, SQ (decreases respiratory secretions, prevents bradycardia) |
For injectable anesthetic guidelines in other species please consult an ARP veterinarian. |
Dilutions of Injectable Anesthetic Mixtures for Use in Rodents
Mouse
Ketamine/Medetomidine
Dosage: |
Ketamine |
75 mg/kg |
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Medetomidine |
1.0 mg/kg |
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Dose Rate: |
0.1 ml/10 gm body weight IP* |
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Volumes: |
Ketamine |
0.375 ml |
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Medetomidine |
0.50 ml |
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WFI** |
4.125 ml |
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Total volume |
5.0 ml = 50 doses |
* IP = intraperitoneal injection ** WFI = water for injection
Ketamine/Xylazine
Dosage: |
Ketamine |
120 mg/kg |
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Xylazine*** |
16.0 mg/kg |
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Dose Rate: |
0.1 ml/10 gm body weight IP* |
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Volumes: |
Ketamine |
0.60 ml |
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Xylazine |
0.40 ml |
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WFI** |
4.00 ml |
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Total volume |
5.0 ml = 50 doses |
* IP = intraperitoneal injection ** WFI = water for injection ***Xylazine in this mixture is at a concentration of 20 mg/ml. Xylazine is available in concentrations of 20 mg/ml and 100 mg/ml.
Rat
Ketamine/Medetomidine
Dosage: |
Ketamine |
75 mg/kg |
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Medetomidine |
0.5 mg/kg |
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Dose Rate: |
0.2 ml/100 gm body weight IP* |
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Volumes: |
Ketamine |
3.0 ml |
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Medetomidine |
0.1 ml |
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WFI** |
4.9 ml |
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Total volume |
8.0 ml = 40 doses |
* IP = intraperitoneal injection ** WFI = water for injection
Ketamine/Xylazine
Dosage: |
Ketamine |
100 mg/kg |
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Xylazine*** |
10 mg/kg |
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Dose Rate: |
0.2 ml/100 gm body weight IP* |
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Volumes: |
Ketamine |
2.0 ml |
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Medetomidine |
1.0 ml |
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WFI** |
1.0 ml |
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Total volume |
4.0 ml = 20 doses |
* IP = intraperitoneal injection ** WFI = water for injection ***Xylazine in this mixture is at a concentration of 20 mg/ml. Xylazine is available in concentrations of 20 mg/ml and 100 mg/ml.
Ketamine/Xylazine/Acepromazine
Dosage: |
Ketamine |
50 mg/kg |
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Xylazine*** |
0.5 mg/kg (100 mg/ml) |
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Acepromazine |
1.0 mg/kg |
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Dose Rate: |
0.2 ml/100 gm body weight IP* |
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Volumes: |
Ketamine |
1.0 ml |
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Xylazine |
0.1 ml |
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Acepromazine |
0.2 ml |
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WFI** |
2.7 ml |
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Total volume |
4.0 ml = 20 doses |
* IP = intraperitoneal injection ** WFI = water for injection ***Xylazine in this mixture is at a concentration of 100 mg/ml. Xylazine is available in concentrations of 20 mg/ml and 100 mg/ml.
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