Intellectual Property Office
Non-Confidential Disclosures
“An Inducible Parvovirus B19 Non-Structural Protein Expression System in Baculovirus Platforms”
PSU Invention Disclosure Number 2006-3216
Field of the Invention/Key Words:
Biomedical Research Tool
Inventors:
Stanley J. Naides, M.D., Leona Gilbert, Ph.D., Viola Kivovich
Background:
Helicases utilize the energy of ATP hydrolysis to translocate along DNA and RNA, simultaneously unwinding double-stranded regions. Parvovirus helicases additionally often possess endonuclease activity, and frequently serve as a scaffold for directing the encapsulation of viral DNA. It is believed that these additional features of parvovirus helicases may play a critical role in human pathogenesis. Unfortunately, the expression of parvovirus helicase in mammalian cells is typically toxic. Consequently, the expression of the human parvovirus B19 helicase nonstructural protein has been shown to induce cell death via host cell DNA damage and subsequent apoptosis. The lack of an effective expression system has made investigations into the role of parvovirus helicase in pathogenesis especially difficult.
Invention description:
We have overcome many of the afore-mentioned complications with our inducible Parvovirus B19 non-structural protein expression system. This invention overcomes the inherent toxic effects of B19 NS1, enabling adequate expression in cell culture systems as well as precise control over the expression of NS1 protein in transformed cells. Additionally, our system is capable of producing a full length NS1 product that is suitable for the preparation of immune polyvalent serum, as well as monoclonal antibodies.
Advantages:
- Able to express full length NS1 protein and deletion mutants.
- Provides a Baculovirus delivery system and cell selective delivery of NS1.
- Permits precise pharmacological control of NS1 expression.
Contact:
James F. Kolonay
Licensing Officer
Intellectual Property Office
The Pennsylvania State University
113 Technology Center
University Park, PA 16802-7000
Phone: (814) 863-7070
Fax: (814) 865-3591
E-mail: jfk11@psu.edu
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